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|a 10.3886/ICPSR38608.v1
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|a DNA Contamination, Degradation, Damage and Associated Microbiomes: A Comparative Analysis Through Massive Parallel Sequencing and Capillary Electrophoresis, United States, 2019-2021
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|a <p>This project evaluated whether DNA contamination can mimic the characteristics of low copy number (LCN), aged, damaged, and degraded DNA samples. Massive Parallel Sequencing/Next Generation Sequencing (MPS/NGS) was used to see if specific patterns of nucleotide damage is present with surface DNA contamination that has been aged and/or exposed to varying concentrations of sodium hypochlorite (bleach) and ultraviolet (UV). The project included two phases. <ul><li>Phase 1.0: Understand the process and rate of degradation and damage of applied touch DNA contamination on human skeletal remains and evidence tape. Five time intervals (ranging from 0 days to 1 year), three bleach treatments, and three UV treatments were tested. Two additional handlers (cumulative) created a scenario of minor contributors often encountered in forensic scenarios.</li> <li>Phase 2.0: Understand the utility and degradation of the skin microbiome associated with touch DNA. This included determining if unique forensic signatures can be identified and compared, especially on bone substrates that may have their own microbiome signature.</li></ul></p>
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